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Fig. 6 | Microbial Cell Factories

Fig. 6

From: bZIP transcription factors PcYap1 and PcRsmA link oxidative stress response to secondary metabolism and development in Penicillium chrysogenum

Fig. 6

Effect of PcYap1 and PcRsmA on oxidative stress response. Effect of Pc-yap1 and Pc-rsmA silencing (A) and overexpression (B) on the amount of naturally occurring and H2O2-generated ROS in mycelium from submerged cultures. Strain C1 contains the empty pGpdPki-RNAi vector used for gene silencing and strain C2 (pyrG) contains the empty pBKSpyrG vector used for gene overexpression. Results are expressed in relative fluorescence units (RFU) per gram of biomass (dry weight) (see “Materials and methods” section for details). C Effect of Pc-yap1 and Pc-rsmA silencing on the viability of conidia submitted to oxidative stress with different concentrations of H2O2. Results are expressed as percentage of conidia able to form colonies on plates with respect to the initial number counted under the microscope (see “Materials and methods” section for details). In all cases bar sizes are the result of three biological replicas, error bars correspond to standard deviation

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