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Fig. 3 | Microbial Cell Factories

Fig. 3

From: bZIP transcription factors PcYap1 and PcRsmA link oxidative stress response to secondary metabolism and development in Penicillium chrysogenum

Fig. 3

Penicillin production in strains with knocked down expression of Pc-yap1 and Pc-rsmA (A) and strains overexpressing the respective genes (B); strain C1 contains the empty pGpdPki-RNAi vector used for gene silencing and strain C2 (pyrG) contains the empty pBKSpyrG vector used for gene overexpression. To test the effect of H2O2-generated oxidative stress, 100 mM H2O2 was added at the beginning of the cultivation time. In the panel at the right in A the extra bar (violet color) corresponds to the parental strain Wis54-1255 grown on medium without added H2O2. Bar sizes are the result of three biological replicas, error bars correspond to standard deviation. See “Materials and methods” section for details

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Microbial Cell Factories

ISSN: 1475-2859

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