Fig. 2

EMSA of PcYap1 binding activity in the pcbAB-pcbC intergenic region. A Positive binding reaction of PcYap1 with the probe PTA1-WT containing the regulatory sequence TTAGTAA, and lack of binding reaction with the probe PTA1-M1 containing the mutant sequence CTAGTCA. B Binding reactions of PcYap1 and PcRsmA with the probes PTA1-WT and PTA1-M1. C Specificity test for the binding of PcYap1 to the TTAGTAA sequence. PTA-WT* is the labelled probe; PTA-WT and PTA-M1 are cold (unlabelled) probes, which were added in excess amounts of 50×, 100×, 150× and 200× as indicated at the top of the gel image. D Screening of the entire pcbAB-pcbC intergenic region to locate target sites of PcYap1; lanes 1 through 6 correspond to binding reactions between probes 1 through 6 with PcYap1, lane F5 is the free probe 5. E Location in the pcbAB gene promoter of the six probes used in D; below is indicated the position and the sequence of the upper strand of probes PTA1-WT and PTA1-M1