Table 3 Plasmids used in this study
From: Engineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules
Plasmid | Description | References |
|---|---|---|
pECAS9-gRNA-kanMX-tHFD1 | 2μ vector with kanMX marker expressing eCas9 under the TEF1 promoter and CYC1 terminator and the gRNA targeting HFD1 under the SNR52 promoter | [51] |
pECAS9-gRNA-kanMX-tPEP4 | 2μ vector with kanMX marker expressing eCas9 under the TEF1 promoter and CYC1 terminator and the gRNA targeting PEP4 under the SNR52 promoter | This study |
pECAS9-gRNA-kanMX-tPRC1 | 2μ vector with kanMX marker expressing eCas9 under the TEF1 promoter and CYC1 terminator and the gRNA targeting PRC1 under the SNR52 promoter | This study |
pECAS9-gRNA-kanMX-tPRB1 | 2μ vector with kanMX marker expressing eCas9 under the TEF1 promoter and CYC1 terminator and the gRNA targeting PRB1 under the SNR52 promoter | This study |
pNatAmyCPOT | 2μ vector with cassette expressing POT1 gene from S. pombe and an expression cassette with α-leader sequence and α-amylase gene under native TPI1 promoter and terminator | [21] |
pCPOT | 2μ vector with cassette expressing POT1 gene from S. pombe and an expression cassette with native TPI1 promoter and terminator without recombinant protein gene | [21] |
pNatZACPOT | 2μ vector with cassette expressing POT1 gene from S. pombe and an expression cassette with α-leader sequence and ZHER3_1-ABD gene under native TPI1 promoter and terminator | This study |
pNatZZACPOT | 2μ vector with cassette expressing POT1 gene from S. pombe and an expression cassette with α-leader sequence and ZHER3_1-ZHER3_1-ABD gene under native TPI1 promoter and terminator | This study |
pNatZAZCPOT | 2μ vector with cassette expressing POT1 gene from S. pombe and an expression cassette with α-leader sequence and ZHER3_1-ABD-ZHER3_1 gene under native TPI1 promoter and terminator | This study |