Table 1 Bivalent binding kinetics assay parameters of ZHER3_1-ABD-ZHER3_1 produced by B184 pep4Δ and by the original host E. coli
From: Engineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules
Production host | ka1 (1/Ms) | ka2 (1/RUs) | kd1 (1/s) | kd2 (1/s) | KD1 (M) | KD2 (M) |
|---|---|---|---|---|---|---|
E. coli | 5.21 × 105 | 1.23 × 10–3 | 1.43 × 10–2 | 2.29 × 10–5 | 2.75 × 10–8 | 1.85 × 10–2 |
S. cerevisiae | 3.83 × 105 | 1.71 × 10–3 | 1.79 × 10–2 | 2.76 × 10–5 | 4.68 × 10–8 | 1.62 × 10–2 |