Fig. 7
From: Engineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules
Fed-batch fermentation of B184 pep4Δ expressing ZHER3_1-ABD-ZHER3_1. Experimental data from a fed-batch bioreactor experiment of B184 pep4Δ expressing ZHER3_1-ABD-ZHER3_1. The cells were cultured in SD2xSCAA medium and condensed feed media. Details of the feed media and strategy are presented in the materials and methods section. A Affibody concentration, cell dry weight (CDW) and glucose addition to the bioreactors. The quantity of the Affibody molecule concentration is based on western blot analysis against the albumin binding domain. All the data are based on biological triplicates and the average and standard deviations are shown. B Metabolic byproduct concentrations in the supernatant during the fed-batch cultivation. All the data are based on biological triplicates and the average and standard deviations are shown. C Affibody titers after the batch phase, after the low-glucose feed phase and at the end of the fed-batch fermentation. This quantification was done using BLI determination for ZHER3_1-ABD-ZHER3_1. The data are based on biological triplicates and technical duplicates and the average and standard deviations are shown