Fig. 4
From: Engineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules
Western blot analysis of the Affibody molecules produced in B184 and B184 pep4Δ. Western blot against the Z-domain (cropped) (A) and against ABD (cropped) (B) in the supernatant of B184 and B184 pep4Δ expressing the three Affibody molecule genes after 96 h of cultivation. For the western blot against the Z-domain, the membrane was blotted with anti-Z-domain antibody (1:1000) followed by an anti-Mouse (1:5000) secondary antibody. For the Western blot against ABD, the membrane was blotted with anti-ABD antibody (3:1000) followed by an anti-Rabbit (1:5000) secondary antibody. The lane marked with st1 contains the standard of ZHER3_1-ABD (10 mg/L) in water, the lane marked with st2 contains the standard of ZHER3_1-ABD-ZHER3_1 (10 mg/L) in water and the lane marked with em contains empty SD2xSCAA media