Fig. 4

Quantitative RT-PCR analysis of the iol transcripts. Strains MK244 and MR01 were grown with (MI +) and without (MI –) 10 mM myo-inositol to extract the total RNA, which was subjected to quantitative RT-PCR to quantify the transcripts of the two tandem iol operons. The latter and former operons contain gk1894 (A) and gk1899 (B) as the first open reading frames, respectively, transcripts of which were quantified and normalized using the comparative C_t method with the internal control of gk0103 transcript. The values are mean ± SD from three independent experiments