Fig. 2
CFUs of transconjugants (solid), donors (open), and recipients (gray). Donor strain YNB213 and recipient strain KV7 were mixed at a 4:1 ratio of recipient: donor for the conjugative transfer of chromosomal segment containing the ΔiolQGK::kan cassette. YNB213 was grown in the presence of IPTG before the conjugation. After keeping the mixed donor and recipient for conjugation, the mixture was allowed to grow with shaking for the time indicated. Colonies of transconjugants, donors, and recipients were counted to calculate CFU. The values are mean ± SD from three independent experiments