Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene

Table 3 Proteomic profiles of ribosomal proteins and translation of S. cerevisiae strains BY4742, BY4742 + pLJ529-ZNF1, and the bud21∆ + pLJ529-ZNF1 during fermentation of mixed 0.05% glucose (w/v) with or without 2% xylose (w/v) at 48 h

Description/strain		Protein fold-change in xylose versus no xylose condition
Protein	Function	BY4742	BY4742	bud21∆	p-value
Protein	Function	BY4742	+ pLJ529-ZNF1	+ pLJ529-ZNF1	p-value
Ribosomal protein and translation
Tma19	Protein that associates with ribosomes	0.99	0.65	2.96	< 0.05
Eft1	Elongation factor 2	0.78	0.44	2.2	< 0.05
Gcd11	Translation initiation factor eIF2 subunit gamma	0.78	0.44	2.2	< 0.05
YNL208W	Hypothetical protein interacts with ribosomes	1.24	1.28	1.69	< 0.05
Dug1	Metallodipeptidase	0.86	0.84	2.62	< 0.05
Efb1	Translation elongation factor 1 subunit beta	0.73	0.23	6.02	< 0.05
Cam1	Translation elongation factor EF1B gamma	1.15	0.73	2.64	< 0.05
Arc1	Protein that binds tRNA	1.4	1.07	3.2	< 0.05
Rim1	Single-stranded DNA-binding protein	1.15	1.9	2.06	< 0.05
Pab1	Polyadenylate-binding protein	0.94	0.98	1.51	< 0.05
Rps0-30	Ribosomal 40S subunit protein A and B	0.24–2.04	0.37–1.42	0.40–4.70	< 0.05
Rpl1-43	Ribosomal 60S subunit protein A and B	0.45–1.33	0.44–2.02	0.56–2.69	< 0.05
Rpp0-1	Ribosomal protein P0 and P1B	1.09	1.11	2.83–5.08	< 0.05
Tef1	Translation elongation factor EF-1 alpha	1.05	0.89	1.75	< 0.05
Yef3	Translation elongation factor EF-3	1.05	0.89	2.66	< 0.05
Tef4	Translation elongation factor EF1B gamma	0.97	0.73	2.6	< 0.05
Tif1	Translation initiation factor eIF4A	0.97	0.73	2.37	< 0.05
Rps31	Ubiquitin-ribosomal 40S subunit	1.11	0.97	1.72	< 0.05
Rpl40	Ubiquitin-ribosomal 60S subunit A and B	1.11	0.97	2.61	< 0.05

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