Fig. 6From: Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 geneSolid state fermentation of rice straw pretreated with Xylaria sp. BCC1067 for xylose to xylitol conversion. A Enzyme activity of cellulase and xylanase (U/g) and sugar concentration (mg/g of rice straw) under solid-state fermentation. Rice straw was pretreated with fungi Xylaria sp. BCC1067 cultivated for 28 days at 25 °C at 70% moisture content for 28 days. The reaction mixture was incubated at 50 °C for 10 min. The reducing sugars released were quantified using glucose or xylose as a standard. B Conversion of xylose to xylitol from rice straw hydrolysate. The BY4742 + pRS316, the BY4742 + pLJ529-ZNF1, the bud21∆ + pRS316, and the bud21∆ + pLJ529-ZNF1 S. cerevisiae strains were grown using rice straw hydrolysate supplemented with YP medium and 0.05% glucose. Strains were incubated at 30 °C with shaking for 60 h. Glucose, xylose and xylitol concentrations were determined by HPLC and CDW (mg/L) was also obtained. Error bars indicated standard deviations calculated from at least two independent experiments performed in triplicate. Significance differences were determined by one-way ANOVA with Tukey HSD method (*, p < 0.05; **, p < 0.01) or (¥, p < 0.05) as compared to BY4742 + pRS316 or bud21∆ + pRS316, respectivelyBack to article page