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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene

Fig. 6

Solid state fermentation of rice straw pretreated with Xylaria sp. BCC1067 for xylose to xylitol conversion. A Enzyme activity of cellulase and xylanase (U/g) and sugar concentration (mg/g of rice straw) under solid-state fermentation. Rice straw was pretreated with fungi Xylaria sp. BCC1067 cultivated for 28 days at 25 °C at 70% moisture content for 28 days. The reaction mixture was incubated at 50 °C for 10 min. The reducing sugars released were quantified using glucose or xylose as a standard. B Conversion of xylose to xylitol from rice straw hydrolysate. The BY4742 + pRS316, the BY4742 + pLJ529-ZNF1, the bud21∆ + pRS316, and the bud21∆ + pLJ529-ZNF1 S. cerevisiae strains were grown using rice straw hydrolysate supplemented with YP medium and 0.05% glucose. Strains were incubated at 30 °C with shaking for 60 h. Glucose, xylose and xylitol concentrations were determined by HPLC and CDW (mg/L) was also obtained. Error bars indicated standard deviations calculated from at least two independent experiments performed in triplicate. Significance differences were determined by one-way ANOVA with Tukey HSD method (*, p < 0.05; **, p < 0.01) or (¥, p < 0.05) as compared to BY4742 + pRS316 or bud21∆ + pRS316, respectively

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