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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene

Fig. 5Fig. 5

Overexpression of ZNF1 and deletion of BUD21 genes conferred tolerance to furfural and lignocellulosic acids stress. The S. cerevisiae wild-type BY4742, the znf1∆, the BY4742 + pRS316, the BY4742 + pLJ529-ZNF1, the bud21∆ + pRS316 and bud21∆ + pLJ529-ZNF1 strains were examined for growth and cell survival. A Growth assays were conducted. Cells were grown in YPX10 media containing 10% xylose (w/v) and 0.05% glucose plus 20 mM furfural (FF), 40 mM formic acid (FA), or 85 mM levulinic acid (LA). Growth of strains were monitored and expressed as the optical density values (OD600) for 5 days at 30 °C. B Spot tests of different S. cerevisiae strains were examined on YPX10 plates containing10% xylose (w/v) and 0.05% glucose to monitor cell survival in the presence of 35 mM formic acid, 20 mM furfural, or 85 mM levulinic acid. Ten-fold serial dilutions of cells were spotted on plates and incubated at 30 °C for 2–3 days. C Cell survival was analyzed using CFU/ml method. Significance differences were determined by one-way ANOVA with Tukey HSD method (*, p < 0.05; **, p < 0.01) as compared to the controls BY4742 or BY4742 + pRS316. Error bars indicated standard deviation (SD)

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