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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene

Fig. 4

Xylose fermentation profile and xylitol production of S. cerevisiae wild type and engineered strains. Different S. cerevisiae strains of BY4742 + pRS316, BY4742 + pLJ529-ZNF1, bud21∆ + pRS316, and bud21∆ + pLJ529-ZNF1 were grown under YPX supplemented with A 2% (w/v) or B 10% (w/v) of xylose mixed with 0.05% glucose at 30 °C for 10 or 18 days, respectively. For all plots presented, xylose concentration (g/L) (solid line), xylitol concentration (g/L) (dashed line). Xylose consumption and xylitol concentrations were determined by HPLC, and data was based on two independent experiments conducted in triplicate. Error bars indicated standard deviations calculated from at least two independent experiments performed in triplicate. Significance differences were determined by one-way ANOVA with Tukey HSD method (*, p < 0.05; **, p < 0.01, as compared to the control BY4742 + pRS316 strain or ¥, p < 0.05 as compared to the control bud21∆ + pRS316)

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Microbial Cell Factories

ISSN: 1475-2859

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