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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene

Fig. 2Fig. 2

Expression levels of genes involved in xylose metabolism in the ZNF1 overexpressing strain (ZNF1-OE) during the growth in 2% xylose and 0.05% glucose mix. A Relative expression levels of xylose metabolic genes (GCY1, GRE3, YDL124W, YPR1, XYL2, SOR1, SOR2 and BUD21 genes) B glycolytic and alcoholic fermentative genes (HXK1, HXK2, GPM1, ENO1, PYK1, PDC1, and ADH1 genes) C hexose transporter and PPP genes (HXT4, HXT7, GAL2, ZWF1, GND1, and TAL1 genes) in ZNF1-OE strain compared to the wild type strain during the growth in 2% xylose and 0.05% glucose mix. The relative expression levels were obtained via the comparative Ct method for quantification of the ∆∆Ct values. Altered expression levels more than 2-folds were considered significant. The average values were calculated from at least two independent experiments performed in three replicates. D Metabolic engineering strategy via overexpression of ZNF1 transcription factor gene to activate its target genes linked to xylose metabolism and deletion of xylose suppressor BUD21 in S. cerevisiae. The green arrow indicated induction of genes expressed by fold-changes which is compared to the wild-type BY4742 strain

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Microbial Cell Factories

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