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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene

Fig. 1

Expression levels of Znf1 target genes during glucose–xylose shift. Relative expression levels of genes involved in xylose metabolism, pentose phosphate pathway, glycolysis, gluconeogenesis, glycerol metabolism and TCA cycle were examined using RT-qPCR. Changes in the levels of mRNAs were indicated as following: 2% xylose shift in the wild-type strain (the X rectangle box), in the wild-type strain relative to the znf1∆ strain during the 2% xylose shift (the Y rectangle box) and, in the wild-type strain relative to the znf1∆ strain during growth in 2% glucose (the Z rectangle box). Green, red or yellow color box indicated genes whose expression was either activated/induced, repressed or unaltered by xylose or the transcription factor Znf1, respectively. The relative expression levels were obtained via the comparative Ct method for quantification of the ∆∆Ct values. Altered expression more than 2-folds was considered significant. The arrowheads in the figure represent the direction of enzymatic reactions. Abbreviations: ADH1, alcohol dehydrogenase I; ADH2, alcohol dehydrogenase II; BUD21, component of small ribosomal subunit/ xylose suppressor; COX1, cytochrome c oxidase; CYC3, cytochrome c heme lyase; ENO1, enolase; FBA1, fructose 1,6-bisphosphate aldolase; GCY1, glycerol dehydrogenase; GDH2, glutamate dehydrogenase; GLK1, glucokinase; GND1, 6-phosphogluconate dehydrogenase; GPD1, glycerol-3-P dehydrogenase; GPD2, glycerol-3-P dehydrogenase; GPM1, phosphoglycerate mutase; GPP1, glycerol-3-P phosphatase; GPP2, glycerol-3-P phosphatase; GRE3, aldose reductase; HXK1, hexokinase isoenzyme 1; HXK2, hexokinase isoenzyme II; PFK1, phosphofructokinase I; PFK2, phosphofructokinase II; PGI1, phosphoglucose isomerase; PGK1, 3-phosphoglycerate kinase; PDC1, pyruvate decarboxylase; PYC1, pyruvate carboxylase; PYK1, pyruvate kinase; RKI1, ribose-5-phosphate ketol-isomerase; RPE1, ribulose-5-phosphate 3-epimerase; SDH1, succinate dehydrogenase; SOL3, 6-phosphogluconolactonase; SOL4, 6-phosphogluconolactonase; SOR1, sorbitol dehydrogenase; SOR2, sorbitol dehydrogenase; TAL1, transaldolase; TDH1, glyceraldehyde-3-phosphate dehydrogenase; TKL1, transketolase; TKL2, transketolase; TPI1, triose phosphate isomerase; XKS1, xylulokinase; XYL2, xylitol dehydrogenase; YDL124W, NADPH-dependent alpha-keto amide reductase; YJR096W, xylose and arabinose reductase; YPR1, NADPH-dependent aldo–keto reductase; ZWF1, glucose-6-phosphate dehydrogenase. Compounds, AKG, α-ketoglutarate; 13BPG, 1,3-bisphosphoglycerate; CIT, citrate; DHAP, dihydroxyacetone phosphate; E4P, erythrose 4-phosphate; FDP, fructose-1,6-diphosphate; F6G, fructose-6-phosphate; FUM, fumarate; GAP, glyceraldehyde-3-phosphate; G6P, glucose-6-phosphate; G3P, glyceraldehyde-3-phosphate; ICI, isocitrate; PEP, phosphoenolpyruvate; 3PG, 3-phosphoglycerate; 6PGC, 6-phosphogluconate; 6PGL, 6-phospho-gluconolactonase; MAL, malate; OXA, oxaloacetate; R5P, ribose 5-phosphate; RL5P, ribulose 5-phosphate; S7P; sedoheptulose 7-phosphate; SUCCoA, succinyl CoA; SUCC, succinate; X5P, xylulose 5-phosphate

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