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Table 1 CAR2 disruption efficiency obtained from combinatorial optimization of Cas9 and gRNA promoters for high-efficiency gene disruption, screening 2 random Cas9 integrants per Cas9 promoter tested

From: Metabolic engineering of Rhodotorula toruloides IFO0880 improves C16 and C18 fatty alcohol production from synthetic media

 

tRNATyr (%)

5S-tRNATyr

pGAPDH-SpCas9

2

n.d

13

p27-SpCas9

13

17%

9

10%

pTEF1-SpCas9

93

97%

96

85%

pANT1-SpCas9

0

100%

2

100%

p17-SpCas9

100

100%

4

9%

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Microbial Cell Factories

ISSN: 1475-2859

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