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Table 1 CAR2 disruption efficiency obtained from combinatorial optimization of Cas9 and gRNA promoters for high-efficiency gene disruption, screening 2 random Cas9 integrants per Cas9 promoter tested

From: Metabolic engineering of Rhodotorula toruloides IFO0880 improves C16 and C18 fatty alcohol production from synthetic media

  tRNATyr (%) 5S-tRNATyr
pGAPDH-SpCas9 2 n.d
13
p27-SpCas9 13 17%
9 10%
pTEF1-SpCas9 93 97%
96 85%
pANT1-SpCas9 0 100%
2 100%
p17-SpCas9 100 100%
4 9%