Fig. 2

Schematic overview of the strain engineering process employed in this study. SpCas9 and MaFAR were co-integrated to create a platform strain, 880CF, followed by integration of gRNA cassettes to create knockout mutants and gene overexpression cassettes for overexpression strains. neo gene encoding aminoglycoside 3'-phosphotransferase (G418 resistance), hpt gene encoding hygromycin phosphotransferase (hygromycin resistance), nat gene encoding nourseothricin acetyltransferase (nourseothricin resistance)