Fig. 5
From: Construction of cell factory capable of efficiently converting l-tryptophan into 5-hydroxytryptamine
The effects of induction temperature, induction time, IPTG concentration and catalysis temperature on 5-HT synthesis. a Optimal L-Trp concentration for 5-HT synthesis. b Optimal culture temperature for 5-HT synthesis. c Optimal IPTG concentration for 5-HT synthesis. d Optimal induction time for 5-HT synthesis. e Optimal catalysis temperature for 5-HT synthesis. Aliquots of solution were taken and diluted for HPLC analysis every 12 h