Fig. 4From: Construction of cell factory capable of efficiently converting l-tryptophan into 5-hydroxytryptamineEnzyme cascade of SmTPH and HaDDC to produce 5-HT in vivo. (a) Protein expression in E. coli BL21(DE3)△tnaA/SmTPH-HaDDC/BH4 and E. coli BL21(DE3)△tnaA/HaDDC-SmTPH/BH4. Lane 1. marker; Lanes 2 & 3. soluble and inclusion expression of E. coli BL21(DE3)△tnaA/SmTPH-HaDDC/BH4; Lanes 4 & 5, soluble and inclusion expression of E. coli BL21(DE3)△tnaA/HaDDC-SmTPH/BH4. (b) Batch production of 5-HT using E. coli BL21(DE3)△tnaA/HaDDC-SmTPH/BH4Back to article page