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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Construction of cell factory capable of efficiently converting l-tryptophan into 5-hydroxytryptamine

Fig. 4

Enzyme cascade of SmTPH and HaDDC to produce 5-HT in vivo. (a) Protein expression in E. coli BL21(DE3)tnaA/SmTPH-HaDDC/BH4 and E. coli BL21(DE3)tnaA/HaDDC-SmTPH/BH4. Lane 1. marker; Lanes 2 & 3. soluble and inclusion expression of E. coli BL21(DE3)tnaA/SmTPH-HaDDC/BH4; Lanes 4 & 5, soluble and inclusion expression of E. coli BL21(DE3)tnaA/HaDDC-SmTPH/BH4. (b) Batch production of 5-HT using E. coli BL21(DE3)tnaA/HaDDC-SmTPH/BH4

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