Fig. 4

Effect of different physical and chemical treatments on anti-mycobacterial property of F-1. a Proteolytic enzymes treatment—C: untreated F-1 (control); 1: treated with α-chymotrypsin; 2: treated with Proteinase K; b heat treatment—C: untreated F-1 (control); 1: treated for 30 min; 2: treated for 60 min; c pH treatment—1: treated with buffer of pH 3.0; 2: treated with buffer of pH 4.5; 3: treated with buffer of pH 6.0; 4: treated with buffer of pH 7.5; 5: treated with buffer of pH 9.0; 6: treated with buffer of pH 10.5; C: untreated F-1 (control)