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Fig. 5 | Microbial Cell Factories

Fig. 5

From: T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation

Fig. 5

Media composition effect on the cultures’ growth kinetics. The growth kinetics of the E. coli BL21(DE3) [pET21d(+)-gfpuv], E. coli BL21(DE3) [pET21d(+)-tthHB27IRM] and E. coli BL21(DE3) [pET21d(+)-tp84_28] bacterial cultures in M9 minimal medium and LB media supplemented with different peptones was measured as OD600. The cultures were carried out at 37 °C. No gene expression-inducing factors were added during culturing

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