Fig. 3
Expression leakage of TP-84 endolysin in E. coli BL21(DE3) [pET21d(+)-tp84_28] cells on the media with various compositions. The cultures were carried out as for E. coli BL21(DE3) [pET21d(+)-gfpuv]. The bacterial cells were lysed and analysed on 10% SDS-PAGE. (Lane M) PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific); (Lane K) purified recombinant TP84_28 protein; (Lane T0) E. coli BL21(DE3) [pET21d(+)-tp84_28] cells at OD600  = 0.6–0.8; (Lane T1) cells 1 h after the culture reached OD600  = 0.6–0.8; (Lane T2) 2 h; (Lane T3) 3 h; (Lane T4) 4 h; (Lane T5) 5 h; (land T6) 6 h; and (Lane TO/N) cells after overnight cultivation. The arrows indicate the position (44.2 kDa) at which endolysin migrates in the gel. The red arrow indicates TP84_28 in the samples containing the expressed tp84_28 gene