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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Recombinant production of the lantibiotic nisin using Corynebacterium glutamicum in a two-step process

Fig. 5

Purification of a His6-tagged soluble variant of NisP. A Immobilized metal affinity chromatography (IMAC) of crude extracts of E. coli BL21/pEKEx-snisP-His6. Indicated is absorbance at 214 nm (red, left y-axis) and % of elution buffer (%B, black, right y-axis) over the elution volume. B Western blot analysis after SDS-PAGE using α-His6 antibody. Samples taken at different steps of the purification of sNisP-His6. CE: crude extract; FT: flowthrough of IMAC; E: eluate of IMAC peak fraction; M: molecular weight marker

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Microbial Cell Factories

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