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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Recombinant production of the lantibiotic nisin using Corynebacterium glutamicum in a two-step process

Fig. 2

Production of prenisin using C. glutamicum and activation of prenisin to nisin culture supernatants. A Growth of C. glutamicum CR099/pXMJ-nisZBTCCg and CR099/pXMJ19 on 2xTY medium with 2% glucose and induction with IPTG (0.2 mM) after 2 h of growth. C Relative mCherry fluorescence normalized to OD600 (RFU/OD) of L. lactis NZ9000/pNZ-Pnis-mcherryLl grown o/N in the presence of supernatants of C. glutamicum CR099/pXMJ-nisZBTCg and CR099/pXMJ19 harvested at the indicated time points of the experiment shown in (B). Prior to assays, supernatants were activated by incubation with trypsin (0.5 mg/ml for 3.5 h) and diluted 1:100. Values are mean ± SD of n = 3 independent cultures

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