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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Escherichia coli coculture for de novo production of esters derived of methyl-branched alcohols and multi-methyl branched fatty acids

Fig. 3

MBE and BCA production by the RQ5.1/CB1.1 coculture system. A Representative TLC of three independent experiments showing MBE production by the RQ5.1/CB1.1 coculture system 48 h post induction, varying the inoculation ratio between both strains. Isobutanol was supplemented to the culture medium at a final concentration of 20 mM for bioconversion assays of the RQ5.1 strain (positive control). BCA-MBE, isobutanol and isopentanol-derived MBE; MBE-Et, ethanol derived MBE; MBE-Phe, 2-phenylethanol derived MBE; MAG, monoacylglycerides; DAG, diacylglycerides. B Densitometric analysis of the MBE spots of the TLC. C Quantification of BCA production for each of the cocultures at the indicated time points. Error bars represent the standard deviation of three independent experiments

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