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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Expression of phenylalanine ammonia lyases in Synechocystis sp. PCC 6803 and subsequent improvements of sustainable production of phenylpropanoids

Fig. 5

Productivity and Western Blot of Synechocystis strains overexpressing PAL or TAL from the pEEK3 vector. A Comparison of tCA production in engineered Synechocystis strains EvC, Pc3C, Pc3N, At3C, At3N, Np3C, Np3N, Av3C, Av3N, Ts3C-H87L and Ts3N-H87L. B pCou production in strains Ts3C and Ts3N. Samples of growth medium were taken on day one, three and six. The values are the means of three biological replicates and two technical replicates, error bars represent the standard deviation. A and B are plotted using the data from Table 2. C Western Blot and SDS-PAGE of extracts from engineered Synechocystis strains (EvC, Pc3C, Pc3N, At3C, At3N, Np3C, Np3N, Av3C, Av3N, Ts3C, Ts3N, Ts3C-H87L and Ts3N-H87L) analyzed for presence of PAL proteins. Upper panel displays the Western Blot using anti-Strep antibody; bottom panel shows the SDS-PAGE loaded with 3 µg of soluble fraction from protein crude extract of the different strains

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Microbial Cell Factories

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