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Fig. 12 | Microbial Cell Factories

Fig. 12

From: Expression of phenylalanine ammonia lyases in Synechocystis sp. PCC 6803 and subsequent improvements of sustainable production of phenylpropanoids

Fig. 12

tCA and pCou production and Western Blot of engineered Synechocystis strains during high-density cultivation experiments. A tCA production in engineered Synechocystis strains EvC, PcC, PcN, AtC, AtN, NpC, NpN, AvC, AvN. B pCou production in strains TsC and TsN. Samples of growth medium were taken on day two and four. The values are the means of three biological replicates and two technical replicates, error bars represent the standard deviation. C WesternBlot and SDS-PAGE of extracts from engineered Synechocystis strains (EvC, PcC, PcN, AtC, AtN, NpC, NpN, AvC, AvN, TsC and TsN) analyzed for presence of PAL proteins taken on day 4 of HDC experiment. Upper panel displays the Western Blot using anti-Strep antibody; bottom panel shows the SDS-PAGE loaded with 3 µg of soluble fraction from protein crude extract of the different strains

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Microbial Cell Factories

ISSN: 1475-2859

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