Fig. 4
From: Biosynthesis and metabolic engineering of 1-hydroxyphenazine in Pseudomonas chlororaphis H18
Rational design of the PhzS to improve its activity to PCA. A Docking of the ligand PCA into the PhzS crystal structure. PCA is shown in yellow. B Relative activity of wild type PhzS and mutants to PCA. C HPLC chromatograms of fermentation broth of PH18-1 and PH18-1–2 at 250 nm. The pink line: PH18-1–2; the black line: PH18-1. The data shown in B are from three experiment replicates, and are expressed as the mean value ± SD