Skip to main content

Table 2 Oligonucleotides used in the study

From: Cell factory for γ-aminobutyric acid (GABA) production using Bifidobacterium adolescentis

Primer

Sequence (5’—> 3’)

Template

Purpose

gadBC_OP_Fw

ccagctcttcgACAacctgcccatcgtagc

B. adolescentis 4–2 genomic DNA

Amplify gadBC gene with the original promoter

gadBC_OP_RV

ccagctcttcgCTAtcagtattccggattcactagc

pKKT427 Fw

caagctcttcgTAGgccaccgtcgccaagg

pKKT427

Amplify pKKT427 plasmid backbone

pKKT427 Rv

caagctcttcgTGTgcctgcatgcaagctt

gadBC Fw

ccagctcttcgatgtcagaaacacattccacc

B. adolescentis 4–2 genomic DNA

Amplify gadBC gene without the original promoter

gadBC Rv

caagctcttcgtcagtattccggattcactagc

pKKT427_ter_Fw

ccagctcttcgTGActgactcactgaacgg

pBCMAT_Pgap_TdppA2

Amplify pKKT427 plasmid backbone, including the gap promoter and terminator

pKKT427_pro_rv

ccagctcttcgCATgatgttctccttgggtca

gadB_RT1_Fw

catgttcctgcgtttgggat

B. adolescentis cDNA

gadB quantitative expression

gadB_RT1_Rv

ccgtcgttccacagcgta

gadC_RT1_Fw

cgtcggtttcgtcgctt

gadC quantitative expression

gadC_RT1_Rv

cacaagaatcgcatatgaaacgcta

16srRNA_FW

cacattccaccgttacacc

Normalize gene expression in B. adolescentis

16srRNA_FW

cgttatccggaattattggg

  1. Underlined sequences are SapI recognition site