Table 1 Bifidobacterium strains and promoters used in this study
From: Cell factory for γ-aminobutyric acid (GABA) production using Bifidobacterium adolescentis
Strain/promoter/plasmid | Characteristic feature/origin | References | |
|---|---|---|---|
Bacterial Strains | B. adolescentis 4-2 | Wild type GABA producer, isolated from adult human feces living in Japan | [22] |
B. adolescentis JCM 1275 | Low transformation efficiency, high oxygen sensitivity, isolated from adult human feces, obtained from JCM company | [19] | |
B. longum 105-A | High transformation efficiency, isolated from adult human gut | [25] | |
B. longum subsp. infantis JCM 1222 | High oxygen sensitivity, isolated from infant gut | [26] | |
B. minimum JCM 5821 | Unique oxygen tolerance, isolated from sewage | [26] | |
E. coli TOP10 | Chemically competent cells | ||
Promoters | Pgap | The promoter of Glyceraldehydes-3-phosphate dehydrogenase gene, it is a part of B. longum 105-A genome | [23] |
PBLt43 | The promoter of tRNA gene, it is a part of B. longum NCC2705 genome | [24] | |
Pori | The promoter of glutamate decarboxylase gene, it is a part of B. adolescentis 4-2 genome | This study | |
Plasmids | pKKT427 | A shuttle vector between Escherachia coli and Bifidobacterium with Spr, modified of pBRATA101 | [19] |
pBCMAT_Pgap_TdppA2 | A plasmid construct for Chloramphenicol assay based on pKKT427 backbone, including gap-promoter | [24] | |
pBCMAT_PBLt43_TdppA2 | A plasmid construct for Chloramphenicol assay based on pKKT427 backbone, including Blt43-promoter | [24] | |
pKKT427::Pori_gadBC | pKKT427 carrying gadB and gadC genes with the original promoter gadB gene | This study | |
pKKT427::Pgap_gadBC | pKKT427 carrying gadB and gadC genes with the gap-promoter | This study | |
pKKT427::PBLt43_gadBC | pKKT427 carrying gadB and gadC genes with Blt43-promoter | This study | |
pPAM1233-1283 | pBAD33 carrying BAD_1233 and BAD_1283 PAM plasmid, used for methylation of pKKT427 | [19] |