Fig. 1
From: Cell factory for γ-aminobutyric acid (GABA) production using Bifidobacterium adolescentis
Cloning plasmid and GABA production by each Bifidobacterium recombinant. A Diagram of expression vector construction displaying the backbone of pKKT427, a Bifidobacterium-E. coli shuttle vector, in which GABA-producing genes were inserted within the multiple cloning site (MCS). The names of three plasmid constructs with different promoters are mentioned in the upper part of the MCS. B Glutamate/GABA conversion by B. adolescentis strains; B. adolescentis 4–2 wild (ado.4–2), B. adolescentis JCM 1275/pKKT427::POri-gadBC (ado-J-ori), B. adolescentis JCM 1275/pKKT427::POri-gadBC (ado-J-gap), B. adolescentis JCM 1275/pKKT427::PBlt43-gadBC (ado-J-Blt43). C, D Bacterial growth and glutamate/GABA conversion pattern in two recombinant strains. C B. adolescentis JCM 1275/pKKT427::POri-gadBC. D B. adolescentis JCM 1275/pKKT427::Pgap-gadBC. Bacterial growth (optical density, OD600; ●), GABA production (mM; ■), and glutamate concentration (mM; ○) are displayed. Values are presented as the means ± SD. Analysis was performed using three independent bacterial cultures