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Table 1 Acceptor proteins quantities produced in the same E. coli SDB1 background but with the pgl locus in plasmid (SDB1 pACYCpgl) or inserted in the chromosome of SDB1 (SDB1pgl) at 30 °C and 37 °C (X indicates ORF inserted coding for G-SodB/G-FlpA/G-NetB/G-ExoA)

From: PglB function and glycosylation efficiency is temperature dependent when the pgl locus is integrated in the Escherichia coli chromosome

Temperature (°C)

E. coli background

Acceptor proteins

G-SodB

G-FlpA

G-NetB

G-ExoA

30

SDB1 pACYCpgl pEXT20_X

0.4

0.92

0.40

1.79

SDB1pgl pEXT20_X

0.24

0.90

0.28

0.83

37

SDB1 pACYCpgl pEXT20_X

0.54

0.41

0.28

0.71

SDB1pgl pEXT20_X

0.45

1.09

0.28

0.55

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Microbial Cell Factories

ISSN: 1475-2859

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