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Fig. 4 | Microbial Cell Factories

Fig. 4

From: PglB function and glycosylation efficiency is temperature dependent when the pgl locus is integrated in the Escherichia coli chromosome

Fig. 4

Comparison between glycosylation of carrier proteins at 30 °C in the presence and absence of additional inducible PglB. Glycosylation without additional inducible PglB of carriers G-SodB (lane 1), G-FlpA (lane2), G-NetB (lane 3) and G-ExoA (lane 4), followed by the effect of addition of inducible PglB in glycosylation of the same carriers at 30 °C, G-SodB (lane 5), G-FlpA (lane 6), G-NetB (lane 7) and G-ExoA (lane 8). 2 µg of each protein was loaded per well. In red is the anti-His and in green the anti-SBA signals

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