Fig. 2From: PglB function and glycosylation efficiency is temperature dependent when the pgl locus is integrated in the Escherichia coli chromosomeDepiction of the modifications performed in the carrier proteins to become suitable PglB substrates (addition of different numbers of non-natural glycotags (2, 8 and 10), PelB or DsbA leaders for co-localisation in the periplasm, and poly-histidine tag for purification as well as detoxifying mutations on both G-ExoA and G-NetB)Back to article page