Table 1 Primers used in this study
Primers | Nucleotide sequence (5′ to 3′) |
|---|---|
cdna1-1UF (hphR) | CCGTCACCAGCCCTGGGTTGCAGACAATGATGGTAGCAGCG |
cdna1-2UF (hphR) | CCGTCACCAGCCCTGGGTTGCTCAAACAGCCTCATGGTTCCCAGA |
cdna1-3UF (hphR) | CCGTCACCAGCCCTGGGTTGGCCGATTCCTTTCCCGGGATGCTCC |
cdna1-4UF (hphR) | CCGTCACCAGCCCTGGGTTGTAGCGAGCGGCCATTTGGAGGTCAT |
cdna1-5UF (hphR) | CCGTCACCAGCCCTGGGTTGGCTTGGGTTGGAAGCGATTCCAGGT |
cdna1-UR (cbh1F) | ATGACGGCCAACTTCCGATACATGTTGAGAGAAGTTGTTGGATTGAT |
cbh1-UF | TACGCCACTGTGAGGAGGCC |
cbh1-UR (hphF) | TTCAATATCAGTTAAGGTCGTCGGTGAGCCACGTGCTTTTT |
cbh1-F | ATGTATCGGAAGTTGGCCGTCAT |
cbh1-R | TTACAGGCACTGAGAGTAGTAAGG |
hph-F | CGACCTTAACTGATATTGAA |
hph-R | CAACCCAGGGCTGGTGACGG |
cbh1-1307UF | AAAGCGTTCCGTCGCAGTAG |
cbh1-724UR | AAGCCGTTGGCAAATTAC |
cbh1-677UF (cbh1-724UR) | AAGCCGTTGGCAAATTACCTTCACTCAGTCCAATCTCA |
cbh1-UR (egfpF) | CTCGCCCTTGCTCACCATGATGCGCAGTCCGCGGTT |
cbh1c-UF | GGTGAAGTGAAAGAAGACAGAGGT |
cbh1-869UF | ACCTGTAAAGCCGCAATG |
egfp-F | ATGGTGAGCAAGGGCGAG |
egfp-R (TtrpCS) | TTTGATGATTTCAGTAACGTTAAGTTTACTTGTACAGCTCGTCCA |
TtrpC-S | ACTTAACGTTACTGAAATCATCAAA |
TtrpC-A | GAGTGGAGATGTGGAGTGGG |
cbh1-621UR | GGAACAAACAAGCGACCC |
cdna1-3UF (cbh1-621UR) | GGGTCGCTTGTTTGTTCCGCCGATTCCTTTCCCGGGATGCTCC |
cdna1-1UF | CAGACAATGATGGTAGCAGCG |
cdna1-UR (cbh1sp) | AGCACGAGCTGTGGCCAAGAAGGCCGAGATGACGGCCAACTTCCGATACATGTTGAGAGAAGTTGTTGGATTGAT |
cdna1-UR (egfpF) | CTCGCCCTTGCTCACCATGTTGAGAGAAGTTGTTGGATTGATC |
bglA-F (cbh1spR) | CTTGGCCACAGCTCGTGCTGTACGTGCCGTCACTTCCTT |
bglA-R (Txyn1S) | CAACATCAACAGAACCTAGTTGCTTTAGTGAACAGTAGGCAGAGACG |
eg2-F (cbh1spR) | CTTGGCCACAGCTCGTGCTCAGCAGACTGTCTGGGGCC |
eg2-R (Txyn1S) | CAACATCAACAGAACCTAGTTGCTCTACTTTCTTGCGAGACACGA |
Txyn1-S | AGCAACTAGGTTCTGTTGATGTTG |
Txyn1-A | GAATAGCCAGAGGACGGTTG |
ptrA-S | AACAAAGATGCAAGAGCGG |
ptrA-A (cbh1-869UF) | CCGCTCTTGCATCTTTGTTACCTGTAAAGCCGCAATG |