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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues

Fig. 5

Expression of bglA driven by Pcc resulted in increase of BGL activity and FPA. Transformants expressing bglA driven by Pcc and the parental strain QP4 cultured on glucose-esculin plates and CMC-esculin plates at 30℃ for 2 days (a). SDS-PAGE analysis of the total extracellular cellulases of transformants and QP4 were shown after cultured in GMM for 5 days (b) and CPM for 7 days (c). BGLA bands were indicated by arrows. BGL activity (d) and FPA (e) of the transformants and QP4 were measured after cultured in GMM for 5 days and CPM for 7 days. Data indicates the mean of three independent experiments; error bars express the standard deviations. Different lowercase letters above the bars indicate significant differences at P < 0.05

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