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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues

Fig. 3

Identification of Pcbh1 activation region (AR) using egfp as the reporter gene. a Strategies of the construction of Pcbh1-dc (Pcbh1 devoid of Cre1-binding sites) and Pcbh1-cr (core promoter of Pcbh1) fused with egfp. RR: regulation region; CR: core region; AR: activation region. b, c Fluorescence relative ratio between the transformants PBD1 (Pcbh1-dc-egfp), PBC1 (Pcbh1-cr-egfp) and the parental strain QP4 cultured in GMM for 2 days (b) and AMM for 3 days (c). Data indicates the mean of three independent experiments; error bars express the standard deviations. Different lowercase letters above the bars indicate significant differences at P < 0.05

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