Table 2 Stability of CB6-Csn after treating with thermal stability, enzymes, pH and organic reagent
Factors | Time (min) | Residual activity (%) |
|---|---|---|
Temperature | ||
Positive control | 60 | 100 |
40 °C | 60 | 100 |
50 °C | 60 | 100 |
60 °C | 60 | 83.33 |
70 °C | 60 | 73.33 |
80 °C | 60 | 73.33 |
90 °C | 60 | 60 |
100 °C | 60 | 40 |
121 °C | 15 | 10.10 |
pH | ||
Positive control | 30 | 100 |
2 | 30 | 92 |
3 | 30 | 94 |
4 | 30 | 94 |
5 | 30 | 98 |
6 | 30 | 100 |
7 | 30 | 100 |
8 | 30 | 80 |
9 | 30 | 80 |
10 | 30 | 68 |
11 | 30 | 68 |
12 | 30 | 52 |
Enzymes | ||
Positive control | 30 | 100 |
Catalase | 30 | 100 |
Pepsin | 30 | 50 |
Proteinase-K | 30 | 66.67 |
Trypsin | 30 | 83.33 |
Papain | 30 | 66.67 |
Organic reagent | ||
Positive control | 60 | 100 |
Isopropanol | 60 | 100 |
Acetone | 60 | 100 |
Methanol | 60 | 100 |
Tween-20 | 60 | 100 |
Tween-80 | 60 | 100 |
EDTA | 60 | 100 |
Acetonitrile | 60 | 100 |