A novel protein fusion partner, carbohydrate-binding module family 66, to enhance heterologous protein expression in Escherichia coli

Table 2 Primers used in this study

Amplicon	Primer	Sequence (5’ to 3’)
CBM66	F1	AGAAGGAGATATACATATGGGAACGACACCT
CBM66	R1	GTAACGAAGGAGTCTCTCGAGCACCACCAC
CBM66-L^a	F2	GTAACGAAGGAGTCTGGTGGCGGAGGCAGCGGTGGAGGCGGATCC
CBM66-L^a	R2	ATCGTCATCGTCACCGGATCCGCCTCCACCGCTGCCTCCGCCACC
CBM66-L-EK^b	F3	GGTGGAGGCGGATCCGGTGACGATGACGATAAGXXXXXXXXXXXXXXX
CBM66-L-EK^b	R3	XXXXXXXXXXXXXXXTCAGTGGTGGTGGTGGTGGTGCTCGAG
pMAL-L-EK	F4	GGAGATATACATATGAAAATAAAAACAGGT
pMAL-L-EK	R4	GCTGCCTCCGCCACCAGTCTGCGCGTCTTT
pGST-L-EK	F5	GGAGATATACATATGTCCCCTATACTAGGT
pGST-L-EK	R5	GCTGCCTCCGCCACCTTTTGGAGGATGGTC
pLE	F6	AGAAGGAGATATACATATGGGTGGCGGAGGC

^aL, flexible linker domain [double repeat of four glycine and one serine, (G₄S)₂]
^bEK, enterokinase cleavage site
X, nucleotides of target genes

ISSN: 1475-2859