|
Tag
|
Protein
|
Origin
|
Size (kDa)
|
Purpose
|
References
|
|---|
|
MBP
|
Maltose-binding protein
|
Escherichia coli
|
43
|
B
|
[48]
|
|
GST
|
Glutathione-S-transferase
|
Schistosoma japonicum
|
27
|
B
|
[49]
|
|
Trx
|
Thioredoxin
|
E. coli
|
12
|
S
|
[50]
|
|
NusA
|
N-utilization substance
|
E. coli
|
54
|
S
|
[51]
|
|
SUMO
|
Small ubiquitin-modified
|
Homo sapiens
|
11
|
S
|
[52]
|
|
6 × His
|
Hexa-histidine
|
n/a
|
< 1
|
A
|
[53]
|
|
FLAG
|
FLAG-octapeptide
|
n/a
|
< 1
|
A
|
[54]
|
|
STREP
|
Streptavidin binding peptide
|
n/a
|
< 1
|
A
|
[55]
|
|
CBM66
|
Carbohydrate-binding module 66
|
Bacillus subtilis
|
18
|
B
|
This study
|
- n/a not applicable, B bifunctional, S soluble expression, A affinity purification
