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Table 1 General fusion tags used in recombinant protein production

From: A novel protein fusion partner, carbohydrate-binding module family 66, to enhance heterologous protein expression in Escherichia coli

Tag Protein Origin Size (kDa) Purpose References
MBP Maltose-binding protein Escherichia coli 43 B [48]
GST Glutathione-S-transferase Schistosoma japonicum 27 B [49]
Trx Thioredoxin E. coli 12 S [50]
NusA N-utilization substance E. coli 54 S [51]
SUMO Small ubiquitin-modified Homo sapiens 11 S [52]
6 × His Hexa-histidine n/a  < 1 A [53]
FLAG FLAG-octapeptide n/a  < 1 A [54]
STREP Streptavidin binding peptide n/a  < 1 A [55]
CBM66 Carbohydrate-binding module 66 Bacillus subtilis 18 B This study
  1. n/a not applicable, B bifunctional, S soluble expression, A affinity purification