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Fig. 4 | Microbial Cell Factories

Fig. 4

From: A novel protein fusion partner, carbohydrate-binding module family 66, to enhance heterologous protein expression in Escherichia coli

Fig. 4

Purification of intact protein from the fusion product. a SDS-PAGE analysis of purification steps. The CBM66 tag and PETase cleaved by enterokinase (lane 1); PETase collected from flow through (lane 2). The CBM66 tag eluted with 50 mM and 100 mM sodium chloride solutions (lanes 3 and 4, respectively). b Bioactivity of PETase samples during the purification steps (intact, CBM66-fused, and purified PETase). Bioactivity of two CBM66-fused model proteins (EGF and ADH1) were compared with commercial enzymes on c and d, respectively. All images are representative of experiments performed in triplicate; all data are expressed as the mean ± standard deviation

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Microbial Cell Factories

ISSN: 1475-2859

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