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Fig. 2 | Microbial Cell Factories

Fig. 2

From: A novel protein fusion partner, carbohydrate-binding module family 66, to enhance heterologous protein expression in Escherichia coli

Fig. 2

Soluble expression with the CBM66 tag. Expression profiles of seven target proteins analyzed using a SDS-PAGE and b western blotting. M, molecular marker; S, soluble protein; I, insoluble; ( −), without CBM66 tag; ( +), with CBM66 tag. c Quantification of expression profiles. The amount of soluble and insoluble proteins are represented as black and white bars, respectively. All images are representative of experiments performed in triplicate; all data are expressed as the mean ± standard deviation

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