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Fig. 1 | Microbial Cell Factories

Fig. 1

From: A novel protein fusion partner, carbohydrate-binding module family 66, to enhance heterologous protein expression in Escherichia coli

Fig. 1

Map of the constructed protein expression plasmid harboring CBM66 fusion tag. The target protein was designed to be expressed with a CBM66 tag on the N-terminus and a His-tag on the C-terminus under the T7 promoter. The flexible linker between CBM66 and the passenger protein comprised a double repeat of four glycine and one serine [(G4S)2]. EK site indicates enterokinase recognition sequence (D4K)

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Microbial Cell Factories

ISSN: 1475-2859

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