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Table 1 Bacterial strains and plasmids used in this study

From: Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineering

Strain or plasmid Relevant genotype or descriptionsa Reference or source
Strains   
 168 trpC2 [44]
 Dpr9 trpC2 Δepr ΔwprA Δmpr ΔnprB Δbpr ΔnprE Δvpr ΔaprE ΔaprX This study
 ΔbdbDC trpC2 bdbDC::spc This study
Plasmids   
 pUC118-CmrΔaprX Plasmid for markerless deletion of aprX gene, containing cat This study
 pHY300PLK Shuttle vector for E. coli and B. subtilis, containing amp and tet Takara Bio
 pHY-S237 pHY300PLK carrying egl-237 with egl-237 promoter This study
 pHY-S237sBLP pHY300PLK carrying the signal, pro and mature sequence of blp fused to the promoter and signal sequence of egl-237 This study
 pHY-BLP01 pHY300PLK carrying the signal, pro and mature sequence of blp fused to the promoter of egl-237 This study
 pHY-BLP02 pHY300PLK carrying the pro and mature sequence of blp fused to the promoter and signal sequence of egl-237 This study
 pHY-BLP03 pHY300PLK carrying the mature sequence of blp fused to the promoter and signal sequence of egl-237 This study
  1. aAntibiotic resistance genes are abbreviated in the table as follows: spc, spectinomycin; cat, chloramphenicol; amp, ampicillin; tet, tetracycline