Strain or plasmid | Relevant genotype or descriptionsa | Reference or source |
---|---|---|
Strains | Â | Â |
 168 | trpC2 | [44] |
 Dpr9 | trpC2 Δepr ΔwprA Δmpr ΔnprB Δbpr ΔnprE Δvpr ΔaprE ΔaprX | This study |
 ΔbdbDC | trpC2 bdbDC::spc | This study |
Plasmids | Â | Â |
 pUC118-CmrΔaprX | Plasmid for markerless deletion of aprX gene, containing cat | This study |
 pHY300PLK | Shuttle vector for E. coli and B. subtilis, containing amp and tet | Takara Bio |
 pHY-S237 | pHY300PLK carrying egl-237 with egl-237 promoter | This study |
 pHY-S237sBLP | pHY300PLK carrying the signal, pro and mature sequence of blp fused to the promoter and signal sequence of egl-237 | This study |
 pHY-BLP01 | pHY300PLK carrying the signal, pro and mature sequence of blp fused to the promoter of egl-237 | This study |
 pHY-BLP02 | pHY300PLK carrying the pro and mature sequence of blp fused to the promoter and signal sequence of egl-237 | This study |
 pHY-BLP03 | pHY300PLK carrying the mature sequence of blp fused to the promoter and signal sequence of egl-237 | This study |