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Table 1 Bacterial strains and plasmids used in this study

From: Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineering

Strain or plasmid

Relevant genotype or descriptionsa

Reference or source

Strains

  

 168

trpC2

[44]

 Dpr9

trpC2 Δepr ΔwprA Δmpr ΔnprB Δbpr ΔnprE Δvpr ΔaprE ΔaprX

This study

 ΔbdbDC

trpC2 bdbDC::spc

This study

Plasmids

  

 pUC118-CmrΔaprX

Plasmid for markerless deletion of aprX gene, containing cat

This study

 pHY300PLK

Shuttle vector for E. coli and B. subtilis, containing amp and tet

Takara Bio

 pHY-S237

pHY300PLK carrying egl-237 with egl-237 promoter

This study

 pHY-S237sBLP

pHY300PLK carrying the signal, pro and mature sequence of blp fused to the promoter and signal sequence of egl-237

This study

 pHY-BLP01

pHY300PLK carrying the signal, pro and mature sequence of blp fused to the promoter of egl-237

This study

 pHY-BLP02

pHY300PLK carrying the pro and mature sequence of blp fused to the promoter and signal sequence of egl-237

This study

 pHY-BLP03

pHY300PLK carrying the mature sequence of blp fused to the promoter and signal sequence of egl-237

This study

  1. aAntibiotic resistance genes are abbreviated in the table as follows: spc, spectinomycin; cat, chloramphenicol; amp, ampicillin; tet, tetracycline