Fig. 7From: Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineeringMBC of wild-type BLP and Q116H mutant against S. aureus. Bactericidal assay against S. aureus NCTC8325 and S. aureus ATCC43300. The enzyme concentrations at which no colonies formed are plotted on the x-axis, noted as N.D. (not detected), and connected to the curve at the next lower concentration by a dotted line. The results presented are the means of three individual experiments. Error bars represent the standard errors of the means. The minimum bactericidal concentration (MBC) of each enzyme is listed in the upper right corner of the plot areaBack to article page