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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineering

Fig. 5

Homology modeling of mature BLP. Ribbon diagram of a predicted BLP structure. The catalytic residues (His22, Asp36, His82, His121, His123) are shown in ball-and-stick format. The zinc ion is shown as a blue ball. Loops 1 to 3, which form the entrance to the catalytic cleft, are shown in yellow in stick format (Loop 1: residues 23–27, Loop 2: residues 113–119, and Loop 3: residues 151–153). Gln116 is shown in orange. The BLP structure was predicted using Discovery Studio (Dassault Systèmes). The template was LasA protease (PDB code 3IT5), which shares 43% amino acid identity with mature BLP. A Cleft side. B Rotated 90° from (A)

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