Fig. 1From: Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineeringSchematic diagram of the ORF region of the expression vectors of BLP. In the figure, “N” and “C” indicate the N-terminus and C-terminus of the protein, respectively, and the number of the amino acid residues in each sequence is given in parentheses. The open reading frame (ORF) of pHY-BLP01 encodes the full-length beta-lytic protease (BLP) protein, including the signal, pro, and mature sequence. The ORF of pHY-BLP02 encodes the pro and mature sequence of BLP fused to the signal sequence of Egl-237. The ORF of pHY-BLP03 encodes the mature sequence of BLP fused to the signal sequence of Egl-237. Each ORF was placed downstream of the egl-237 promoter and cloned into pHY300PLKBack to article page