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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineering

Fig. 1

Schematic diagram of the ORF region of the expression vectors of BLP. In the figure, “N” and “C” indicate the N-terminus and C-terminus of the protein, respectively, and the number of the amino acid residues in each sequence is given in parentheses. The open reading frame (ORF) of pHY-BLP01 encodes the full-length beta-lytic protease (BLP) protein, including the signal, pro, and mature sequence. The ORF of pHY-BLP02 encodes the pro and mature sequence of BLP fused to the signal sequence of Egl-237. The ORF of pHY-BLP03 encodes the mature sequence of BLP fused to the signal sequence of Egl-237. Each ORF was placed downstream of the egl-237 promoter and cloned into pHY300PLK

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