Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids

Table 4 Removing the feedback inhibition and transcriptional attenuation of other enzymes

Host	Mutation sites	Titer of BCAAs (shake flask) (g/L)	Titer of BCAAs (fed batch) (g/L)	Productivity (g/(L × h))	Yield (g/g glucose)	Substrate	References
C. glutamicum DSM8890	V323A TD	l-Isoleucine: 16.4				Glucose	[68]
C. glutamicum IWJ001	P176S/D426E/L575W AHAS, F383V TD	l-Isoleucine: 5.83				Glucose	[69]
C. glutamicum JHI3-156	P176S/D426E/L575W AHAS, F383V TD	l-Isoleucine: 5.77	l-Isoleucine: 30.7	0.426	0.120	Glucose	[70]
C. glutamicum ATCC13869	A279T AK, G378S HD, F383V TD	l-Isoleucine: 3.53				Glucose	[71]
C. glutamicum ATCC14067	V140M/F383A TD	l-Isoleucine: 0.73				Glucose	[72]
C. glutamicum ML1-9	R529H/G532D/L535V IPMS		l-Leucine: 38.1	0.794		Glucose	[64]
C. glutamicum KCCM11662P	R558H/G561D IPMS	l-Leucine: 13.1				Glucose	[67]
C. glutamicum ATCC13032	G20D/I21D/I22F AHAS, R529H/G532D IPMS	l-Leucine: 6.82	l-Leucine: 23.7	0.564	0.219	Glucose	[33]
C. glutamicum ATCC13032	Y553D IPMS	l-Leucine: 1.55				Glucose	[66]

ISSN: 1475-2859