Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids

Yu, Shengzhu; Zheng, Bo; Chen, Zhenya; Huo, Yi-Xin

doi:10.1186/s12934-021-01721-0

Microbial Cell Factories

Table 3 Enhancement of BCAAs biosynthetic pathways

From: Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids

Host	Overexpression genes	Knockout genes	Titer of BCAAs (shake flask) (g/L)	Titer of BCAAs (fed batch) (g/L)	Productivity (g/(L × h))	Yield (g/g glucose)	Substrate	References
C. glutamicum R	ilvBN^G156E, ilvC^{S34G/L48E/R49F}, ilvD, leuDH	ΔldhA	l-Valine: 227		7.17	0.410	Glucose	[52]
C. glutamicum R JCM 18229	ilvBN^G156E, ilvC^{S34G/L48E/R49F}, ilvD, leuDH, gapA, pyk, pfk, pgi, tpi	ΔldhA, Δppc, Δpta, ΔackA, ΔctfA, ΔavtA	l-Valine: 150			0.573	Glucose	[53]
C. glutamicum ATCC13032	ilvN ^{G20D/I21D/I22F}, replacing ilvD promoter with P-ilvDM7, replacing ilvE promoter with P-ilvEM6	ΔpanB, replacing ilvA promoter with P-ilvAM1CG	l-Valine: 15.9				Glucose	[51]
C. glutamicum ATCC13032	ilvBNC, ilvD	ΔilvA, ΔpanBC	l-Valine: 10.7				Glucose	[49]
C. glutamicum YILW	brnFE	ΔbrnQ		l-Isoleucine: 29.0			Glucose	[54]
C. glutamicum IWJ001	gnd, fbp, pgl		l-Isoleucine: 10.9	l-Isoleucine: 29.0	0.345	0.138	Glucose	[55]
C. glutamicum WM001	phaCAB		l-Isoleucine: 9.58	l-Isoleucine: 29.8		0.129	Glucose	[56]
C. glutamicum JHI3-156	lrp, brnFE		l-Isoleucine: 3.49	l-Isoleucine: 26.9	0.374	0.122	Glucose	[57]
C. glutamicum MH20-22B	ilvA ^V323A, hom ^G378E, introducing the ilvA-con promoter mutation, replacing hom promoter with PcspB	Introducing the PdapA-C13 promoter mutation	l-Isoleucine: 6.95	l-Isoleucine: 14.3	0.184	0.137	Glucose	[58]
C. glutamicum JHI3-156	zwf, ppnK		l-Isoleucine: 4.10		0.057	0.072	Glucose	[59]
C. glutamicum ATCC13869	lysC, hom, thrB, ilvA	Δddh	l-Isoleucine: 3.81				Glucose	[60]
C. glutamicum YILW		ΔalaT	l-Isoleucine: 2.09	l-Isoleucine: 15.4		0.136	Glucose	[61]
C. glutamicum XQ-9	leuA, ilvBN, leuDH, ilvC^{S34G/L48E/R49F}, rocG	ΔltbR, ΔilvE, Δgdh	l-Leucine: 23.3			0.191	Glucose	[62]
C. glutamicum FA-1	aspB	ΔilvE	l-Leucine: 20.8				Glucose	[63]

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