Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids

Table 2 Regulation of central carbon metabolism in BCAAs producing strains

Host	Overexpression genes	Knockout genes	Titer of BCAAs (shake flask) (g/L)	Titer of BCAAs (fed batch) (g/L)	Productivity (g/(L × h))	Yield (g/g glucose)	Substrate	References
C. glutamicum aceE A16	ilvBNC, ilvE	Δpqo, Δppc		l-Valine: 86.5	1.60	0.234	Glucose	[42]
C. glutamicum ATCC13869	ilvBNC, lrp, brnFE	ΔaceE, ΔalaT, ΔilvA	l-Valine: 28.5	l-Valine: 51.2	0.533	0.308	Glucose	[45]
C. glutamicum ATCC13032	ilvBNC, ilvE	ΔaceE, Δpqo, Δpgi		l-Valine: 48.0	0.656	0.488	Glucose, acetate	[41]
C. glutamicum ATCC13032	ilvBNC, ilvE	ΔaceE	l-Valine: 12.3	l-Valine 24.6	1.17	0.391	Glucose, acetate	[40]
C. glutamicum AN02		ΔP_gltA:: P_{CP_2836}	l-Valine: 15.3			0.168	Glucose	[44]
C. glutamicum ATCC13032	ilvBNC, ilvE	ΔaceE, Δpqo	l-Valine: 12.4	l-Valine 24.6		0.150	Glucose, acetate	[46]
C. glutamicum ATCC13032	ilvBNC, ilvE	Δppc, Δpyc, ICD^G407S	l-Valine: 8.90			0.220	Glucose	[47]
C. glutamicum ATCC13032		Δpqo, Δppc, ΔaceE, ΔalaT	l-Valine: 2.64				Glucose	[48]
C. glutamicum XQ-9	ilvBNC, leuA	ΔltbR, Δpyc, ΔavtA, inserting terminator before alaT	l-Leucine: 28.5				Glucose	[43]
C. glutamicum ATCC13032	leuA ^R529H/G532D, ilvN ^{G20D/I21D/I22F}	ΔltbR, ΔleuA, ΔiolR, replacing gltA promoter with P_dapA-L1	l-Leucine: 6.82	l-Leucine: 23.7	0.564	0.219	Glucose	[33]

ISSN: 1475-2859