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Table 2 Regulation of central carbon metabolism in BCAAs producing strains

From: Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids

Host Overexpression genes Knockout genes Titer of BCAAs (shake flask) (g/L) Titer of BCAAs (fed batch) (g/L) Productivity (g/(L × h)) Yield
(g/g glucose)
Substrate References
C. glutamicum aceE A16 ilvBNC, ilvE Δpqo, Δppc   l-Valine: 86.5 1.60 0.234 Glucose [42]
C. glutamicum ATCC13869 ilvBNC, lrp, brnFE ΔaceE, ΔalaT, ΔilvA l-Valine: 28.5 l-Valine: 51.2 0.533 0.308 Glucose [45]
C. glutamicum ATCC13032 ilvBNC, ilvE ΔaceE, Δpqo, Δpgi   l-Valine: 48.0 0.656 0.488 Glucose, acetate [41]
C. glutamicum ATCC13032 ilvBNC, ilvE ΔaceE l-Valine: 12.3 l-Valine 24.6 1.17 0.391 Glucose, acetate [40]
C. glutamicum AN02   ΔPgltA:: PCP_2836 l-Valine: 15.3    0.168 Glucose [44]
C. glutamicum ATCC13032 ilvBNC, ilvE ΔaceE, Δpqo l-Valine: 12.4 l-Valine 24.6   0.150 Glucose, acetate [46]
C. glutamicum ATCC13032 ilvBNC, ilvE Δppc, Δpyc, ICDG407S l-Valine: 8.90    0.220 Glucose [47]
C. glutamicum ATCC13032   Δpqo, Δppc, ΔaceE, ΔalaT l-Valine: 2.64     Glucose [48]
C. glutamicum XQ-9 ilvBNC, leuA ΔltbR, Δpyc, ΔavtA, inserting terminator before alaT l-Leucine: 28.5     Glucose [43]
C. glutamicum ATCC13032 leuA R529H/G532D, ilvN G20D/I21D/I22F ΔltbR, ΔleuA, ΔiolR, replacing gltA promoter with PdapA-L1 l-Leucine: 6.82 l-Leucine: 23.7 0.564 0.219 Glucose [33]